American Society of Microbiology : ABMM Questions & Answers
Updated: Jun 20, 2026
No. of Questions: 204 Questions & Answers with Testing Engine
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American Society of Microbiology ABMM Sample Questions:
1. A microbiology laboratory receives a sample of cerebrospinal fluid from a patient with suspected bacterial meningitis. The Gram stain shows Gram-positive diplococci. Culture on blood agar shows alpha-hemolytic colonies that are bile soluble and optochin-sensitive. The MOST likely organism is:
A) Listeria monocytogenes
B) Streptococcus pneumoniae
C) Enterococcus faecalis
D) Streptococcus agalactiae
2. A patient develops a vesicular rash on their hands and feet, accompanied by fever and oral ulcers. The MOST likely causative agent is:
A) Herpes simplex virus
B) Varicella-zoster virus
C) Coxsackievirus A
D) Measles virus
3. Acyclovir is a commonly used antiviral agent effective against Herpes Simplex Virus (HSV) and Varicella- Zoster Virus (VZV). Its selective toxicity relies on which initial step for activation within infected cells?
A) Blocking viral entry through competitive inhibition of cellular receptors
B) Direct inhibition of viral RNA polymerase activity
C) Monophosphorylation primarily by viral thymidine kinase
D) Phosphorylation by host cell mitochondrial kinases
4. A clinical microbiology laboratory is investigating an outbreak of vancomycin-resistantEnterococcus faecium (VRE) in a hospital. Whole-genome sequencing of isolates from different patients reveals the presence of the vanA operon on a mobile genetic element that is highly conserved across all isolates. This finding MOST strongly suggests:
A) Selection of vancomycin-resistant mutants within a susceptible E. faecium population due to antibiotic pressure.
B) Horizontal transfer of the vanA operon to multiple susceptible E. faecium strains.
C) Clonal spread of a single VRE strain carrying the vanA operon.
D) Independent acquisition of vancomycin resistance by different E. faecium strains.
5. A clinical microbiology laboratory is validating a new multiplex PCR assay for the simultaneous detection of several gastrointestinal pathogens, including Salmonella, Shigella, and Campylobacter. To ensure the accuracy and reliability of the assay, it is crucial to include appropriate controls. Which of the following sets of controls would be MOST comprehensive?
A) Positive controls for each target organism and a negative control without any template DNA.
B) Positive controls for each target organism, a negative control containing non-target DNA, and a process control to monitor nucleic acid extraction.
C) Positive controls for each target organism, a negative control without template DNA, an internal amplification control, and a process control to monitor nucleic acid extraction.
D) Positive controls for each target organism, a negative control without template DNA, and an internal amplification control.
Solutions:
| Question # 1 Answer: B | Question # 2 Answer: C | Question # 3 Answer: C | Question # 4 Answer: C | Question # 5 Answer: C |
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