ASCP : ASCP-MLT Questions & Answers
Updated: Aug 03, 2026
No. of Questions: 562 Questions & Answers with Testing Engine
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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Topic 1: Immunology | 5–10% | - Principles of immunology - Serologic procedures and immune diseases |
| Topic 2: Microbiology | 15–20% | - Virology and parasitology testing - Bacteriology and mycology procedures |
| Topic 3: Chemistry | 20–25% | - Electrolytes, acid-base, and toxicology - Carbohydrates, lipids, and enzymes |
| Topic 4: Urinalysis and Other Body Fluids | 5–10% | - Physical and chemical testing - Microscopic analysis and disease correlation |
| Topic 5: Laboratory Operations | 5–10% | - Quality assessment and safety - Instrumentation and laboratory mathematics |
| Topic 6: Blood Banking | 15–20% | - Blood products and components - Transfusion practice and testing - Blood group systems and immunology |
| Topic 7: Hematology | 20–25% | - Physiology and disease states - Laboratory testing and hemostasis |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
1. Howell-Jolly bodies are composed of DNA and appear as small round ball-like inclusions inside the red cells.
Usually only one Howell-Jolly body will be present in each red cell.
Single erythrocyte inclusions which are large, round, smooth and purplish-blue staining are most likely:
A) Howell-Jolly bodies
B) Heinz bodies
C) Cabot rings
D) Basophilic stippling
2. Failure to deliver glucose drawn in a serum separator tube (SST) to the laboratory within the recommended time will cause:
Question options:
A) a falsely decreased glucose value
B) glycolysis
C) a falsely increased glucose value
D) both A and C
3. The renal threshold is the maximum amount of a substance that the kidney can prevent from entering into the urine.
UA & body fluids
The renal threshold is best described as:
A) Concentration at which a substance in the blood spills into urine
B) Concentration at which kidney failure begins
C) Concentration at which kidney can no longer filter the blood
D) Concentration at which reabsorption first occurs
4. The most common specimen analyzed in the hematology section is:
A) whole blood.
B) plasma.
C) serum.
D) urine.
5. 21 CFR 606.65 states, "Supplies and reagents shall be used in a manner consistent with instructions provided by the manufacturer." The correct answer is A. The reagents must be used according to the manufacturer's instructions for use. If the instructions say one or two drops may be used and the facility procedures also allow one or two drops, then there is no problem with the staff members choosing to use two drops.
While equipment must be calibrated, it does not generally influence the number of drops used.
Staff members often ask co-workers and colleagues from other facilities how to perform tasks, but it is the manufacturer's instructions for the operation of equipment and the use of reagents that must be followed.
A laboratory employee who is performing an internal audit of routine ABO and Rh typing procedures notes that a technologist places two drops of Anti-D in a tube, centrifuges the tube for 20 seconds, and reads the reaction using a magnifying mirror. When questioned about the procedure, the technologist indicates that most staff use two drops of Anti-D reagent because the reactions are stronger with two drops. In addition to reviewing the facility procedure manual, what should be done to ensure regulatory compliance?
A) Review the anti-D manufacturer's current instructions for use.
B) Check the calibration records for the centrifuge.
C) Call a colleague at another institution to ask what they do.
Solutions:
| Question # 1 Answer: A | Question # 2 Answer: D | Question # 3 Answer: A | Question # 4 Answer: A | Question # 5 Answer: A |
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